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External link. The radially aligned 3D scaffold developed through this study has a higher mechanical strength than the previously developed aligned nanofibrous scaffold and native cornea tissue, since the periphery of the 3D radially aligned scaffold is connected to a membrane made of random nanofibers. The nanotopography of the nanofibers can be controlled by simply changing the electrospinning setup such as the applied voltage, solution flow rate, and modification of the collector 2829 Ultimately, we plan to explore seeding and maturation of iPSC-derived renal progenitors in our perfusable 3D PT constructs. Methods Extracellular matrix preparation and rheology The ECM is comprised of a network of gelatin and fibrin.

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Contrary to the 2D controls, we find that enhanced megalin expression is strongly correlated with superior albumin functional uptake in the perfused 3D PTs, suggesting that both their 3D architecture and perfusion improve epithelial function likely due to enhanced cell polarity and brush border Fig.

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Bioprinting of 3D Convoluted Renal Proximal Tubules on Perfusable Chips

AB Fluorescence images comparing the cell migration when rCCs were cultured on the membranes of randomly oriented and radially aligned fibers, respectively, for 8 days. Recently, in the area of tissue engineering, research has focused on developing scaffolds that can repair or replace the functions of damaged tissues or organs. Aquaporin-1 facilitates epithelial cell migration in kidney proximal tubule. PTEC-TERT1 were developed as a cell model that maintains morphological and functional properties of primary PTEC cells with an additional replicative advantage over primary cells that have a finite lifespan in vitro due to telomere shortening 16

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